Test Subject Green

 is a puzzle platformer game released May 10th 2011. The game is a sequel to Test Subject Blue released on March 2nd of the same year. Test Subject Green will retain most elements from Test subject Blue, and will star Blue. Nitrome has said that the game went into production right after Test Subject Blue. From the Trailer released on April 20th, Blue is the hero and Green Enzymes are the enemy.

Controls
Arrow Keys (← ↑ →) - Move

Down Arrow: Duck Space Bar - Fire Enzyme Bullet

Plot
The professor is testing Blue against Green Enzymes. During test 3, the Professor is attacked in his lab. After Blue completes test 3, the Professor puts Blue in test 4, struggling to do so. The test container is knocked over during the fight, and when Blue approaches the Food Pill in the level, he is grabeed by Doctor Nastidous's hand. Blue is then taken to an unnamed area, where Doctor Nastidous tests him against his inventions. After completing 25 test, the Doctor injects Blue into aRex210, where the games ends with "To Be Continued".

Gameplay
Test Subject Green retains the same gameplay as in its predecessor: Obtain the Key Card and get to the Food Pill.

Levels
Test Subject Green has 30 levels, 10 more than its predecessor. 5 are set in the Professor's lab at Xeno Industries, while 25 are set in Doctor Nastidous's Lab.

Crawling Green Enzymes
Green Enzymes which crawl on the ground.

Monkey Green Enzymes
Green Enzymes that have the ability to propel themselves up and move on the ceiling.

Mimic Green Ezymes
Green Enzymes which posses the ability to mimic Blue's very movement.

Lasers
Lasers kill the player if the beam is touched.

April 7th
On April 7th, Nitrome released the below image in a blog post telling fans that Test Subject Green was in development.

April 20th
Nitrome released a 58 second Trailer of Test Subject Green, revealing new features, the storyline, and enemies. -WKrFcHwLbY

Trivia

 * In the ending, Doctor Nastidous opens up a door and places Blue in a suit with two legs and a gun. Blue then walks off the pad he was on, and the door closes with the words "To Be Continued". This ending hints a possibility of Test Subject Blue 3.
 * On level 4, while the Professor is being beaten up, words can be heard in the background.
 * This game, and the other game in the series, are probably both influenced by the Portal series of games.
 * In the preload for the game, a Green Enzyme (possibly Green) is dreaming of him beating Blue with a rolling pin.
 * The Startup and the cutscene seen in the Trailer for the game were both cut from the game for unknown reasons.
 * Test Subject Blue is the second game to be in a trilogy,the first being Ice Breaker.
 * Doctor Nastidous's pda thingy has the Cuboy Logo on the screen.