Walking orange enzymes

 are enemies in Test Subject Blue. They are the basic type of Enzyme, and first Enzyme introduced in the game. Walking Orange Enzymes seem to be squishy, as their body and hands move fluidly around, as if liquid. Walking Orange Enzymes are spawned from a Mine like object, which will spawn the Orange Enzymes one after another in small intervals. will walk completely straight, going through Green Teleporters and falling down long drops. They are the weakest type of Orange Enzyme, as they will die from one shot of Blue's Proton Canon or even touching a wall. Hazards and really long drops will not harm them, just as they won't harm Blue.

Since Orange Enzyme Bullets are the Walking Orange Enzymes pure essence, all Orange Enzymes are immune to them. Usually in one level, there is one Spawn Mine, however, more Spawn Mines will introduced into a single level later in Test Subject Blue. In the ending for Test Subject Blue, what seems to be a Walking Orange Enzyme was merged with a Blue Orange Enzyme.